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Cells were counted to normalize samples, lysed by the addition of deionized water, and centrifuged, and the supernatant was kept at 20C until magnesium determinations in duplicates were made by atomic absorption spectrophotometry (Perkin-Elmer Co)

Hanas R, Lytzen L, Ludvigsson J

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During intestinal inflammation, TJs show strand breaks, and changes in TJ proteins composition and function as well as impaired structure and remodeling of apical junctions (213)

doi: 10.3389/fcell.2023.1269724 24 de FreitasLFHamblinMR

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